In vitro surfactant protein B deficiency inhibits lamellar body formation.
Am J Respir Cell Mol Biol
; 29(2): 259-66, 2003 Aug.
Article
en En
| MEDLINE
| ID: mdl-12649122
ABSTRACT
Surfactant protein (SP) B is essential for normal pulmonary surfactant activity and lamellar body genesis in type 2 cells. However, the role of SP-B in lamellar body genesis is poorly understood. We developed an adenovirus vector expressing antisense SP-B as an alternative in vitro model of SP-B deficiency to begin to explore the role of SP-B in lamellar body genesis. RT-PCR analysis revealed that antisense SP-B expression interfered with translation of endogenous SP-B mRNA. Antisense SP-B expression resulted in reliable in vitro reproduction of many features of SP-B deficiency, including absent mature SP-B and decreased lamellar bodies and SP-C. Light and electron microscopy demonstrated significant reductions in lamellar body number. Western blotting revealed a significant reduction in mature 8-kD SP-B protein and decreased mature SP-C. Our data indicate that antisense SP-B can be effectively used to replicate the SP-B-deficient type 2 cell phenotype in vitro, and provides an attractive alternative to transgenic models for the further study of the role of SP-B in lamellar body genesis.
Search on Google
Banco de datos:
MEDLINE
Asunto principal:
Proteína B Asociada a Surfactante Pulmonar
/
Pulmón
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Año:
2003
Tipo del documento:
Article