Loofa sponge as a scaffold for the culture of human hepatocyte cell line.
Biotechnol Prog
; 19(2): 522-7, 2003.
Article
en En
| MEDLINE
| ID: mdl-12675596
ABSTRACT
Loofa sponge was investigated as a three-dimensional scaffold for stationary and perfusion culture of human hepatoblastoma cell line C3A/HepG2. In stationary culture, C3A/HepG2 cells in loofa cubes showed higher alpha-fetoprotein and albumin secretion rates than those in polyurethane foam (PU). To use loofa cylinders in a packed-bed reactor, immobilization of C3A/HepG2 cells by recirculating medium at 26 mL/min (superficial velocity = 51.7 cm/min) resulted in a cell loading density of 5.15 x 10(7) cells/cm(3)-loofa. This cell loading density is higher than values reported in the literature for packed-bed reactor intended for bioartificial liver. During 9 days of perfusion culture in the reactor, immobilized C3A/HepG2 showed steady synthesis of albumin with an average synthesis rate at 42.2 microg/10(6) cells/day. These experimental results and observations by SEM suggested that loofa sponge is a suitable scaffold for high-density culture of human hepatocyte cell line and the immobilized cells could express high levels of liver-specific functions.
Search on Google
Banco de datos:
MEDLINE
Asunto principal:
Hepatoblastoma
/
Reactores Biológicos
/
Luffa
/
Ingeniería de Tejidos
/
Técnicas de Cultivo
/
Frutas
/
Membranas Artificiales
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Año:
2003
Tipo del documento:
Article