[IgM-ELISA for diagnosis of schistosomiasis mansoni in low endemic areas]. / ELISA-IgM para diagnóstico da esquistossomose mansoni em área de baixa endemicidade.
Cad Saude Publica
; 19(1): 255-61, 2003.
Article
en Pt
| MEDLINE
| ID: mdl-12700805
ABSTRACT
An immunoenzymatic method for the detection of IgM antibodies (IgM-ELISA) against a fraction of Schistosoma mansoni adult worm antigen, soluble in trichloroacetic acid (TCA-soluble fraction), was evaluated for epidemiological purposes in low endemic areas for schistosomiasis. Blood samples on filter paper were collected from a population living in the municipality of Pedro de Toledo, São Paulo State, and submitted to IgM-ELISA. The results were compared to those obtained by the IgM-immunofluorescence test (IgM-IFT) and the Kato-Katz parasitological method. Positive rates of 36.8%, 33.5%, and 1.6% were obtained respectively by the IgM-ELISA, IgM-IFT, and Kato-Katz methods. The geometric mean obtained by the parasitological method was 40.9 eggs per gram of feces (epg). The nearly perfect agreement observed between the two serological tests (Kappa index of 0,89) indicates good diagnostic performance by the evaluated test. IgM-ELISA is a potentially useful method for diagnosis of schistosomiasis in individuals with low worm burden.
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Banco de datos:
MEDLINE
Asunto principal:
Esquistosomiasis mansoni
/
Inmunoglobulina M
/
Anticuerpos Antihelmínticos
/
Enfermedades Endémicas
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Female
/
Humans
/
Male
Idioma:
Pt
Año:
2003
Tipo del documento:
Article