A method of alphavirus replicon particle titration based on expression of functional replicase/transcriptase.
J Virol Methods
; 109(2): 133-8, 2003 May.
Article
en En
| MEDLINE
| ID: mdl-12711055
ABSTRACT
Alphavirus replicon particles are being exploited for a variety of purposes both in vitro as gene expression vectors, and in vivo as vaccines or gene therapy vectors. There is a need for a simple and universal method of titration of replicon particles that is independent of expression of the foreign protein. We devised a method that uses modified vaccinia virus Ankara (MVA) as an indicator virus, to deliver a Venezuelan equine encephalitis virus (VEE) defective helper RNA encoding green fluorescent protein (GFP). Co-infection of cells with the MVA-based indicator and Venezuelan equine encephalitis virus replicon particles (VRP) results in expression of the GFP gene. VRP titer is readily determined by counting fluorescent cells.
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Banco de datos:
MEDLINE
Asunto principal:
Replicón
/
ARN Polimerasas Dirigidas por ADN
/
Virus Defectuosos
/
Virus de la Encefalitis Equina Venezolana
/
Proteínas Luminiscentes
Límite:
Animals
Idioma:
En
Año:
2003
Tipo del documento:
Article