Deformation of intracellular endosomes under a magnetic field.
Eur Biophys J
; 32(7): 655-60, 2003 Nov.
Article
en En
| MEDLINE
| ID: mdl-12811432
ABSTRACT
We present a non-invasive method to monitor the membrane tension of intracellular organelles using a magnetic field as an external control parameter. By exploiting the spontaneous endocytosis of anionic colloidal ferromagnetic nanoparticles, we obtain endosomes possessing a superparamagnetic lumen in eukaryotic cells. Initially flaccid, the endosomal membrane undulates because of thermal fluctuations, restricted in zero field by the resting tension and the curvature energy of the membrane. When submitted to a uniform magnetic field, the magnetized endosomes elongate along the field, resulting in the flattening of the entropic membrane undulations. The quantification of the endosome deformation for different magnetic fields allows in situ measurement of the resting tension and the bending stiffness of the membrane enclosing the intracellular organelle.
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Banco de datos:
MEDLINE
Asunto principal:
Endosomas
/
Compuestos Férricos
/
Membrana Celular
/
Campos Electromagnéticos
/
Fluidez de la Membrana
/
Micromanipulación
Tipo de estudio:
Evaluation_studies
Idioma:
En
Año:
2003
Tipo del documento:
Article