High-performance liquid chromatographic analysis of bioactive triterpenes in Perilla frutescens.
J Pharm Biomed Anal
; 32(6): 1175-9, 2003 Aug 21.
Article
en En
| MEDLINE
| ID: mdl-12907261
ABSTRACT
Perilla frutescens (L.) Britt. (Lamiaceae), a famous traditional Chinese medicine, has been used for the treatment of various diseases. To evaluate the quality of P. frutescens, a simple, rapid and accurate high-performance liquid chromatography (HPLC) method was developed for the assessment of three bioactive triterpene acids tormentic acid (TA), oleanolic acid (OA) and ursolic acid (UA). The HPLC system used an Spherisob octadecylsilyl silica (ODS) column with acetonitrile and aqueous H(3)PO(4) as the mobile phase and detection at 206 nm. The method was precise with relative standard deviations for these three constituents that ranged between 0.6-1.5% (intraday) and 0.7-2.6% (interday). The content of these three phytochemicals in the leaves of P. frutescens growing at eight different locations of China was determined to establish the effectiveness of the method.
Search on Google
Banco de datos:
MEDLINE
Asunto principal:
Triterpenos
/
Cromatografía Líquida de Alta Presión
/
Perilla
Idioma:
En
Año:
2003
Tipo del documento:
Article