c-kit gene expression in CD7-positive acute lymphoblastic leukemia: close correlation with expression of myeloid-associated antigen CD13.
Leukemia
; 6(7): 662-8, 1992 Jul.
Article
en En
| MEDLINE
| ID: mdl-1378163
ABSTRACT
Expression of human c-kit proto-oncogene and interleukin-7 receptor (IL-7R) in acute lymphoblastic leukemia (ALL) cells expressing CD7 was examined by Northern-blot analysis and reversed transcription polymerase chain reaction (RT-PCR) assay in relation to the phenotypes. Leukemic cells from four out of 12 CD7+ ALL patients, all of which fulfilled the criteria of ALL in the FAB classification, expressed c-kit genes. Surface CD3 (sCD3) was absent in all of these cases, while cytoplasmic CD3 (cCD3) was found in the two sCD3- cases. CD3 epsilon transcripts were detected in one of the sCD3- cCD3- cases. IL-7R genes were transcribed in the three cases with c-kit gene expression. In addition, there was a good correlation between c-kit gene expression and myeloid associated antigen CD13 positivity of the leukemic cells. None of the patients with c-kit gene expression had mediastinal tumor. Our results show that leukemic cells in a proportion of CD7+ ALL express receptors for cytokines that are secreted by bone marrow stromal cells. Ligands for c-kit genes and IL-7 could play an important role for the regulation of proliferation and differentiation of T-cell progenitors in bone marrow.
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Banco de datos:
MEDLINE
Asunto principal:
Antígenos de Diferenciación Mielomonocítica
/
Antígenos de Diferenciación de Linfocitos T
/
Antígenos CD
/
Proteínas Proto-Oncogénicas
/
Leucemia-Linfoma Linfoblástico de Células Precursoras
Tipo de estudio:
Risk_factors_studies
Límite:
Humans
Idioma:
En
Año:
1992
Tipo del documento:
Article