The C/EBP family of transcriptional activators is functionally important for Ig VH promoter activity in vivo and in vitro.
J Immunol
; 149(10): 3225-31, 1992 Nov 15.
Article
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| MEDLINE
| ID: mdl-1431100
ABSTRACT
We have examined the functional importance of binding sites for C/EBP family members (E sites), in two Ig VH promoters VH1, a member of the S107 family, and BCL1, a member of the J558 family. Mutation of the E site in the VH1 promoter diminishes transcription in vivo to 59% of wild-type and transcription from the BCL1 promoter in vitro is inhibited to an average of 39% of wild-type by competition with E site oligonucleotides. Purified E site binding proteins from plasmacytoma cells stimulated BCL1 transcription in vitro 2.3-fold. Although five C/EBP family proteins are known which bind the E site, antibody ablation of DNAprotein complexes resolved by electrophoretic mobility shift assays showed that Ig/EBP-1 is the only E site binding protein detectable in early B cell lines; more mature B cells contain Ig/EBP-1 and NF-IL6. We also show by antibody-depletion that Ig/EBP-1 activates the BCL1 promoter in vitro.
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Banco de datos:
MEDLINE
Asunto principal:
Región Variable de Inmunoglobulina
/
Genes de Inmunoglobulinas
/
Transactivadores
/
Regiones Promotoras Genéticas
/
Cadenas Pesadas de Inmunoglobulina
Límite:
Animals
Idioma:
En
Año:
1992
Tipo del documento:
Article