Synthesis and release of acetylcholine in the rabbit kidney cortex.
Life Sci
; 51(22): 1699-703, 1992.
Article
en En
| MEDLINE
| ID: mdl-1435079
ABSTRACT
Several cholinergic processes were demonstrated and partially characterized in rabbit kidney cortical minces choline uptake, acetylcholine synthesis and calcium-dependent release. Minces took up labelled choline, acetylated it, and stored it in a pool that was not readily accessible to physostigmine-sensitive cholinesterase activity. [3H]Acetylcholine synthesis but not [3H]choline uptake was inhibited by the removal of sodium ions or incubation at 0 degrees C. The release of newly synthesized [3H]acetylcholine was increased by 300 mOsmol urea in a calcium-dependent manner, but not by potassium depolarization (300 mOsmol), vasopressin (10 microM), or bradykinin (10 microM). These results suggest that acetylcholine may be synthesized by non-neuronal rabbit kidney cortical cells and that this transmitter may be released in response to physiological levels of urea.
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Banco de datos:
MEDLINE
Asunto principal:
Conejos
/
Acetilcolina
/
Colina
/
Corteza Renal
Límite:
Animals
Idioma:
En
Año:
1992
Tipo del documento:
Article