Isolation of the promoters of Atlantic salmon MHCII genes.
Mar Biotechnol (NY)
; 5(3): 253-60, 2003.
Article
en En
| MEDLINE
| ID: mdl-14502397
ABSTRACT
The major histocompatibility complex class II (MHCII) has a central role in the immune response of vertebrates with its function of presenting antigenic peptides to the T-cell receptors. We have isolated the promoters and intron 1 of MHCIIalpha and MHCIIbeta genes of Atlantic salmon. To isolate these promoters, we constructed an Atlantic salmon ( Salmo salar) promoter finder kit (analogous to the commercially available "human promoter finder kit"). By nucleotide sequence alignment of known MHCII promoter regions, we identified the 3 conserved regulatory X, X2, and Y boxes in the salmon promoters. The W box was not found. In contrast, a salmon-specific putative W box was identified. Both of the isolated Atlantic salmon MHCIIalpha and beta promoters (included in patent applications by Genomar A/S, Oslo, Norway) were found to be functional since they both gave positive yellow fluorescence protein signal when inserted as promoters in the pEYFP-1 reporter plasmid and transfected into the salmon head kidney cell line (SHK-1).
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Banco de datos:
MEDLINE
Asunto principal:
Regiones Promotoras Genéticas
/
Genes MHC Clase II
/
Salmo salar
Límite:
Animals
Idioma:
En
Año:
2003
Tipo del documento:
Article