Vaccinia-expressed human papillomavirus 16 and 18 e6 and e7 as a therapeutic vaccination for vulval and vaginal intraepithelial neoplasia.
Clin Cancer Res
; 9(14): 5205-13, 2003 Nov 01.
Article
en En
| MEDLINE
| ID: mdl-14614000
ABSTRACT
PURPOSE:
Anogenital intraepithelial neoplasia is a chronic disorder associated with infection by high-risk human papillomavirus (HPV) types. It is frequently multifocal and recurrence after conventional treatment is high. Boosting HPV-specific cell-mediated immune responses may reduce progression to carcinoma and could lead to disease clearance. We have tested the safety, immunogenicity, and efficacy of a recombinant vaccinia candidate vaccine (TA-HPV) in women with anogenital intraepithelial neoplasia. EXPERIMENTALDESIGN:
Twelve women, aged 42-54 years with high-grade HPV-positive vulval or vaginal intraepithelial neoplasia of up to 15 years duration, completed a Phase II study of TA-HPV, a live recombinant vaccinia virus, expressing modified versions of the E6 and E7 open reading frames from HPV-16 and HPV-18.RESULTS:
The vaccine was well tolerated. Five of 12 (42%) patients showed at least a 50% reduction in total lesion diameter over 24 weeks with 1 patient showing complete regression of her lesion. Overall, 83% of women showed some improvement with an average decrease in lesion size of 40%. All cases showed an increased IgG titer and T-cell response to the vaccinia virus. An IFN-gamma enzyme-linked immunospot assay using pooled 22-mer peptides spanning HPV-16 E6 and E7 showed an increased specific T-cell response after vaccination in 6 of the 10 cases available for testing. There was no increase in specific cytotoxic response to selected individual HLA class I-restricted HPV-16 E6/7 peptides.CONCLUSIONS:
The results suggest that the vaccine may have an effect on HPV-positive vulval intraepithelial neoplasia/vaginal intraepithelial neoplasia and that additional studies are warranted to develop an effective therapeutic vaccine.
Search on Google
Banco de datos:
MEDLINE
Asunto principal:
Papillomaviridae
/
Proteínas Represoras
/
Virus Vaccinia
/
Neoplasias Vaginales
/
Neoplasias de la Vulva
/
Carcinoma in Situ
/
Vacunas contra el Cáncer
/
Infecciones por Papillomavirus
/
Proteínas de Unión al ADN
Límite:
Adolescent
/
Adult
/
Female
/
Humans
/
Middle aged
Idioma:
En
Año:
2003
Tipo del documento:
Article