[Thymosin beta10 expression and actin filament organization in tumor cell lines with different metastatic potential].
Zhonghua Bing Li Xue Za Zhi
; 33(1): 67-71, 2004 Feb.
Article
en Zh
| MEDLINE
| ID: mdl-14989934
ABSTRACT
OBJECTIVE:
To investigate the expression of thymosin beta10 (Tbeta10) and related changes of actin filament organization in human tumor cell lines with different metastatic potential.METHODS:
Four groups of nine human tumor cell lines with different metastatic potential were analyzed for the expression of Tbeta10 mRNA detected by northern-blot and its peptide by immunohistochemical staining. The filamentous actin (F-actin) was stained with TRITC-phalloidin to detect changes in actin organization.RESULTS:
In comparison with the non and/or weakly metastatic counterparts, Tbeta10 was upregulated in highly metastatic human lung cancer, malignant melanoma and breast cancer cell lines. TRITC-phalloidin staining revealed less actin bundles and a fuzzy network of shorter filaments in the highly metastatic tumor cells, while in the non and/or weakly metastatic cancer cell lines, there were thick and orderly arranged actin filaments.CONCLUSIONS:
Tbeta10 levels correlate positively with the metastatic phenotype in human tumors currently examined. The increased metastatic potential of tumor cells is accompanied by the loss of F-actin and poorly organized actin skeleton. There is a consistent correlation between the elevated Tbeta10 expression and the disrupted actin skeleton.
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Banco de datos:
MEDLINE
Asunto principal:
Timosina
/
Actinas
/
Metástasis de la Neoplasia
Límite:
Humans
Idioma:
Zh
Año:
2004
Tipo del documento:
Article