A simple remedy against artifactual double bands in denaturing gradient gel electrophoresis.
J Microbiol Methods
; 57(2): 279-81, 2004 May.
Article
en En
| MEDLINE
| ID: mdl-15063068
ABSTRACT
Denaturant gradient gel electrophoresis (DGGE) is a widely used method for mutation analysis and for studies of microbial diversity. Particular combinations of target gene fragments and primers may give rise to erroneous DGGE profiles. We report on a very straightforward means to eliminate the artifactual 'double bands' that can be encountered in several applications.
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Banco de datos:
MEDLINE
Asunto principal:
Microbiología del Agua
/
ADN Bacteriano
/
Reacción en Cadena de la Polimerasa
/
Dermatoglifia del ADN
/
Electroforesis en Gel de Poliacrilamida
Idioma:
En
Año:
2004
Tipo del documento:
Article