Patterns of methylation profiles as diagnostic markers for multiple hepatocellular carcinomas.
Scand J Gastroenterol
; 39(3): 246-51, 2004 Mar.
Article
en En
| MEDLINE
| ID: mdl-15074394
ABSTRACT
BACKGROUND:
Hepatocellular carcinoma often displays multiple tumor nodules, thus posing a problem for differential diagnosis between cancers of both multifocal and metastatic origin. Conventionally, pathological criteria have been used for this purpose, but these are largely subjective. In order to facilitate a more objective differential diagnosis of multiple HCCs, we used the patterns of methylation of p16INK4a, p14ARF, and GSTP1 genes as markers for each tumor nodule.METHODS:
Sixty-seven nodules from 30 cases of multiple or recurrent HCCs were examined using methylation-specific PCR (MSP) analysis for the detection of methylation profiles.RESULTS:
Hypermethylation was detected in 56.7%, 43.3% and 17.9% of the cases for p16INK4a, p14ARF, and GSTP1 genes, respectively. At the genetic level the inter-nodule methylation profiles were heterogeneous in 23 of the cases and homogeneous in another 7, enabling a multifocal origin to be diagnosed in the former and metastatic origin in the latter.CONCLUSIONS:
Methylation profiling seems to be useful in differentiating the clonal origins of multiple cancers, as the information yielded by this method is essentially objective.
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Banco de datos:
MEDLINE
Asunto principal:
Aciltransferasas
/
Carcinoma Hepatocelular
/
Metilación de ADN
/
Genes p16
/
Proteína p14ARF Supresora de Tumor
/
Neoplasias Hepáticas
Tipo de estudio:
Diagnostic_studies
Límite:
Female
/
Humans
/
Male
Idioma:
En
Año:
2004
Tipo del documento:
Article