Determination of ephedra alkaloid and caffeine concentrations in dietary supplements and biological fluids.
J Anal Toxicol
; 28(3): 152-9, 2004 Apr.
Article
en En
| MEDLINE
| ID: mdl-15107144
ABSTRACT
Dietary supplements containing botanical forms of caffeine and ephedra alkaloids have been widely promoted and used in the U.S. for weight loss and athletic enhancement despite a lack of adequate research on the pharmacology of these botanical stimulants. In order to analyze dietary supplements and perform human pharmacokinetic studies, an analytical approach with good precision and accuracy was needed with sufficient sensitivity to detect very low levels of ephedra alkaloids. A liquid chromatography-atmospheric pressure chemical ionization (APCI) tandem mass spectrometry (LC-MS-MS) method was developed for quantitating the various ephedrine-group alkaloids found in dietary supplements that contain Ephedra species, and in plasma and urine of persons consuming these supplements. Using this method, low nanogram-per-milliliter concentrations of ephedrine, pseudoephedrine, norephedrine, norpseudoephedrine, methylephedrine, methylpseudoephedrine, and caffeine can be quantitated in a 12-min LC-MS-MS run.
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Banco de datos:
MEDLINE
Asunto principal:
Cafeína
/
Medicamentos Herbarios Chinos
/
Suplementos Dietéticos
/
Alcaloides
/
Efedrina
Límite:
Humans
País como asunto:
America do norte
Idioma:
En
Año:
2004
Tipo del documento:
Article