Selection of mouse cells with amplified metallothionein genes retaining their glucocorticoid inducibility.
FEBS Lett
; 310(1): 75-8, 1992 Sep 21.
Article
en En
| MEDLINE
| ID: mdl-1526285
ABSTRACT
Two new mouse cell mutants, resistant to either 80 or 100 mM CdCl2, were isolated to study the regulation of transcription by the glucocorticoid hormones. Their metallothionein mt-1% and mt-2+ genes were amplified coordinately to a maximum of 30 copies per cell. By Southern blot analysis, no gross rearrangement was detectable near the mt+ loci. Contrary to other mutants previously isolated, the metallothionein-specific mRNAs of these mutants are inducible by dexamethasone.
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Banco de datos:
MEDLINE
Asunto principal:
Dexametasona
/
Metalotioneína
Límite:
Animals
Idioma:
En
Año:
1992
Tipo del documento:
Article