Direct detection of Staphylococcus aureus mRNA using a flow through microarray.
J Microbiol Methods
; 60(1): 47-54, 2005 Jan.
Article
en En
| MEDLINE
| ID: mdl-15567224
ABSTRACT
The direct detection of mRNAs from bacterial cultures on a DNA array without amplification and labelling would greatly extend the range of applications suitable for microarray analysis. Here we describe the direct detection of 23S rRNA and seven mRNA species from total Staphylococcus aureus RNA prepared using commercially available RNA purification columns followed by fluorescent detection on a flow through microarray. RNA hybridisation was detected using paired secondary labelled probes directly 5' and 3' to immobilised 60 mers. In this way, we were able to detect the effect of 30-min exposure to antimicrobials on mRNA levels within 3 h after column purification of total RNA without the need for enzymatic manipulation. Specifically the expression of mecA was confirmed in a highly resistant strain and induction of katA and ile-tRNA synthetase genes after exposure to mupirocin could be detected.
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Banco de datos:
MEDLINE
Asunto principal:
Staphylococcus aureus
/
ARN Bacteriano
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ARN Mensajero
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Análisis de Secuencia por Matrices de Oligonucleótidos
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Año:
2005
Tipo del documento:
Article