Quantitation of surfactant protein B by HPLC in bronchoalveolar lavage fluid.
J Chromatogr B Analyt Technol Biomed Life Sci
; 814(2): 325-30, 2005 Jan 25.
Article
en En
| MEDLINE
| ID: mdl-15639455
ABSTRACT
A sensitive reversed phase HPLC method with evaporative light scattering detection (ELSD) was developed for the determination of the hydrophobic surfactant protein B (SP-B) in human bronchoalveolar lavage fluid. Samples were extracted two times with CHCl(3)MeOHHCl (230.005N) solution in a ratio of 12 by volume. The extract of the lower phase was separated on a C4 butyl silica gel column with an isocratic elution using a mobile phase, consisting of 97% methanol, 2.75% chloroform and 0.25% 0.1 M trifluoroacetic acid (by volume), at a flow rate of 1 ml/min. SP-B was detected by ELSD and quantified by comparison to an external standard. The duration of a run was 7 min, the quantification limit 30 ng and the limit of detection was at about 15 ng of SP-B. This method is suitable for the rapid routine quantification of SP-B in human bronchoalveolar lavage fluid samples.
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Banco de datos:
MEDLINE
Asunto principal:
Líquido del Lavado Bronquioalveolar
/
Cromatografía Líquida de Alta Presión
/
Proteína B Asociada a Surfactante Pulmonar
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Año:
2005
Tipo del documento:
Article