Intracellular multiplication of Legionella pneumophila depends on host cell amino acid transporter SLC1A5.
Mol Microbiol
; 55(5): 1528-37, 2005 Mar.
Article
en En
| MEDLINE
| ID: mdl-15720558
ABSTRACT
The infectious agent of Legionnaires' disease, Legionella (L) pneumophila, multiplies intracellularly in eukaryotic cells. This study has been performed to explore the nutrient requirements of L. pneumophila during intracellular replication. In human monocytes, bacterial replication rate was reduced by 76% in defined medium lacking L-cysteine, L-glutamine or L-serine. SLC1A5 (hATB(0,+)), a neutral amino acid transporter, was upregulated in the host cells after infection with L. pneumophila. Inhibition of SLC1A5 by BCH, a competitive inhibitor of amino acid uptake as well as siRNA silencing of the slc1a5 gene blocked intracellular multiplication of L. pneumophila without compromising viability of host cells. These observations suggest that replication of L. pneumophila depends on the function of host cell SLC1A5.
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Banco de datos:
MEDLINE
Asunto principal:
Monocitos
/
Legionella pneumophila
/
Sistema de Transporte de Aminoácidos ASC
Límite:
Humans
Idioma:
En
Año:
2005
Tipo del documento:
Article