Junctional recruitment of mammalian Scribble relies on E-cadherin engagement.
Oncogene
; 24(27): 4330-9, 2005 Jun 23.
Article
en En
| MEDLINE
| ID: mdl-15806148
ABSTRACT
Members of the LAP protein family, LET-413 in Caenorhabditis elegans, Scribble in Drosophila melanogaster, and Erbin, Lano, Densin-180 and hScrib in mammals, have conserved structural features. LET-413 and Scribble are junctional proteins involved in establishing and maintaining epithelial cell polarity. scribble also behaves as a neoplastic tumor suppressor gene. We show here that, in epithelial cells, hScrib is recruited at cell-cell junctions in an E-cadherin-dependent manner as shown by calcium switch assays in MDCK cells, re-expression of E-cadherin in MDA-231 cells treated by 5-Aza-2'-deoxycytidine (5Aza), and siRNA experiments. hScrib is restricted at the basolateral membrane of epithelial cells by its LRR domain, and is enriched in Triton X-100-insoluble fractions. In breast cancers, most lobular tumors did not express hScrib and E-cadherin while ductal tumors had a less frequent downregulation of hScrib. Our data provide additional insights on the modalities of recruitment of hScrib at the cell-cell junctions, and establish a potential link between the E-cadherin and hScrib tumor suppressors.
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Banco de datos:
MEDLINE
Asunto principal:
Cadherinas
/
Uniones Intercelulares
/
Proteínas de la Membrana
Límite:
Animals
/
Humans
Idioma:
En
Año:
2005
Tipo del documento:
Article