Gene profiling reveals specific oncogenic mechanisms and signaling pathways in oncocytic and papillary thyroid carcinoma.
Oncogene
; 24(25): 4155-61, 2005 Jun 09.
Article
en En
| MEDLINE
| ID: mdl-15806164
ABSTRACT
The oncogenic pathways in mitochondrial-rich thyroid carcinomas are not clearly understood. To investigate the possible implication of mitochondrial abundance in the genesis of thyroid tumors, we have explored the gene expression profile of six oncocytic carcinomas and six mitochondrial-rich papillary carcinomas using cDNA-microarray technology. A supervised approach allowed us to identify 83 genes differentially expressed in the two types of carcinoma. These genes were classified according to their ontologic profiles. Three genes, NOS3, alpha-actinin-2 and alpha-catenin, suspected of playing a role in tumor genesis, were explored by quantitative RT-PCR analysis and immunohistochemistry. Of the 59 genes overexpressed in papillary carcinomas, 51% were involved in cell communication. Of the 24 genes overexpressed in oncocytic carcinomas, 84% were involved in mitochondrial and cellular metabolism. Our results suggest that mitochondrial respiratory chain complexes III and IV play a significant role in the regulation of reactive oxygen species production by oncocytic tumors.
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Banco de datos:
MEDLINE
Asunto principal:
Oncogenes
/
Neoplasias de la Tiroides
/
Carcinoma
/
Carcinoma Papilar
/
Transducción de Señal
/
Perfilación de la Expresión Génica
Límite:
Humans
Idioma:
En
Año:
2005
Tipo del documento:
Article