The rapid isolation and growth dynamics of the tsetse symbiont Sodalis glossinidius.
FEMS Microbiol Lett
; 248(1): 69-74, 2005 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-15961259
ABSTRACT
Sodalis glossinidius is known exclusively in endosymbiosis with tsetse flies (Genus Glossina) and is one of the few insect bacterial symbionts that have been successfully cultured in vitro. This study details improved isolation and solid culture protocols that allow for a standardised and rapid preparation/maintenance of clonal material from individual flies. The isolation and culture of S. glossinidius was confirmed by partial sequencing of the 16S rDNA gene and specific PCR. In addition, the growth dynamics and changes in cell viability during liquid culture are described. The potential for culture of other endosymbiont taxa is discussed.
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Banco de datos:
MEDLINE
Asunto principal:
Simbiosis
/
Moscas Tse-Tse
/
Enterobacteriaceae
Límite:
Animals
Idioma:
En
Año:
2005
Tipo del documento:
Article