Field evaluation of the chessboard modification for serotyping of Streptococcus pneumoniae in a small laboratory in Bangladesh.
Am J Trop Med Hyg
; 74(5): 863-7, 2006 May.
Article
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| MEDLINE
| ID: mdl-16687694
ABSTRACT
The chessboard modification of the quellung method for serotyping Streptococcus pneumoniae (Pneumococcus) was introduced in the Gonoshasthaya Vaccine Research Laboratory, a small laboratory in Bangladesh. We applied initial bench-side training and subsequent continuous surveillance and quality assurance as approaches for good laboratory practice. Results obtained a this laboratory on 1,101 consecutive isolates had satisfactory sensitivity (85.1-100%) and specificity (97.9-99.9%) for serotyping the 10 most common serogroups/types of pneumococci when compared with the results obtained in the Finnish reference laboratory for pneumococcal serotyping at the National Public Health Institute. We conclude that serotyping of pneumococci by the chessboard method can be introduced into a small laboratory by providing basic bacteriologic skills, adequate initial training, and continuous external support.
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Banco de datos:
MEDLINE
Asunto principal:
Infecciones Neumocócicas
/
Garantía de la Calidad de Atención de Salud
/
Streptococcus pneumoniae
/
Serotipificación
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
/
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
/
Screening_studies
Límite:
Adult
/
Child
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Female
/
Humans
/
Male
País como asunto:
Asia
Idioma:
En
Año:
2006
Tipo del documento:
Article