Interactions of ribosomal protein S1 with DsrA and rpoS mRNA.
Biochem Biophys Res Commun
; 348(2): 662-8, 2006 Sep 22.
Article
en En
| MEDLINE
| ID: mdl-16890206
ABSTRACT
Ribosomal protein S1 is shown to interact with the non-coding RNA DsrA and with rpoS mRNA. DsrA is a non-coding RNA that is important in controlling expression of the rpoS gene product in Escherichia coli. Photochemical crosslinking, quadrupole-time of flight tandem mass spectrometry, and peptide sequencing have identified an interaction between DsrA and S1 in the 30S ribosomal subunit. Purified S1 binds both DsrA (K(obs) approximately 6 x 10(6) M(-1)) and rpoS mRNA (K(obs) approximately 3 x 10(7) M(-1)). Ribonuclease probing experiments indicate that S1 binding has a weak but detectable effect on the secondary structure of DsrA or rpoS mRNA.
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Banco de datos:
MEDLINE
Asunto principal:
Proteínas Ribosómicas
/
Factor sigma
/
Proteínas Bacterianas
/
ARN Mensajero
/
ARN no Traducido
/
Proteínas de Escherichia coli
Tipo de estudio:
Prognostic_studies
Idioma:
En
Año:
2006
Tipo del documento:
Article