VE-cadherin-CreERT2 transgenic mouse: a model for inducible recombination in the endothelium.
Dev Dyn
; 235(12): 3413-22, 2006 Dec.
Article
en En
| MEDLINE
| ID: mdl-17072878
ABSTRACT
To introduce temporal control in genetic experiments targeting the endothelium, we established a mouse line expressing tamoxifen-inducible Cre-recombinase (Cre-ERT2) under the regulation of the vascular endothelial cadherin promoter (VECad). Specificity and efficiency of Cre activity was documented by crossing VECad-Cre-ERT2 with the ROSA26R reporter mouse, in which a floxed-stop cassette has been placed upstream of the beta-galactosidase gene. We found that tamoxifen specifically induced widespread recombination in the endothelium of embryonic, neonatal, and adult tissues. Recombination was also documented in tumor-associated vascular beds and in postnatal angiogenesis assays. Furthermore, injection of tamoxifen in adult animals resulted in negligible excision (lower than 0.4%) in the hematopoietic lineage. The VECad-Cre-ERT2 mouse is likely to be a valuable tool to study the function of genes involved in vascular development, homeostasis, and in complex processes involving neoangiogenesis, such as tumor growth.
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Banco de datos:
MEDLINE
Asunto principal:
Endotelio Vascular
/
Antígenos CD
/
Cadherinas
/
Integrasas
Límite:
Animals
/
Pregnancy
Idioma:
En
Año:
2006
Tipo del documento:
Article