Crystallization of ornithine acetyltransferase from yeast by counter-diffusion and preliminary X-ray study.
Acta Crystallogr Sect F Struct Biol Cryst Commun
; 62(Pt 12): 1294-7, 2006 Dec 01.
Article
en En
| MEDLINE
| ID: mdl-17142921
ABSTRACT
A study is presented on the crystallization of ornithine acetyltransferase from yeast, which catalyzes the fifth step in microbial arginine synthesis. The use of the counter-diffusion technique removes the disorder present in one dimension in crystals grown by either the batch or hanging-drop techniques. This makes the difference between useless crystals and crystals that allow successful determination of the structure of the protein. The crystals belong to space group P4, with unit-cell parameters a = b = 66.98, c = 427.09 A, and a data set was collected to 2.76 A.
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Banco de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Acetiltransferasas
Idioma:
En
Año:
2006
Tipo del documento:
Article