Evaluation of neurovirulence and biodistribution of Venezuelan equine encephalitis replicon particles expressing herpes simplex virus type 2 glycoprotein D.
Vaccine
; 25(12): 2296-305, 2007 Mar 08.
Article
en En
| MEDLINE
| ID: mdl-17239997
ABSTRACT
The safety of a propagation-defective Venezuelan equine encephalitis virus (VEEV) replicon particle vaccine was examined in mice. After intracranial inoculation we observed approximately 5% body weight loss, modest inflammatory changes in the brain, genome replication, and foreign gene expression. These changes were transient and significantly less severe than those caused by TC-83, a live-attenuated vaccinal strain of VEEV that has been safely used to immunize military personnel and laboratory workers. Replicon particles injected intramuscularly or intravenously were detected at limited sites 3 days post-administration, and were undetectable by day 22. There was no evidence of dissemination to spinal cord or brain after systemic administration. These results demonstrate that propagation-defective VEEV replicon particles are minimally neurovirulent and lack neuroinvasive potential.
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Banco de datos:
MEDLINE
Asunto principal:
Replicón
/
Proteínas del Envoltorio Viral
/
Virus de la Encefalitis Equina Venezolana
/
Encefalomielitis Equina Venezolana
Límite:
Animals
País como asunto:
America do sul
/
Venezuela
Idioma:
En
Año:
2007
Tipo del documento:
Article