Beta vulgaris L. suspension cultures permeabilized with triton X-100 retain cell viability and betacyanines production ability: a digital image analysis study.
Biotechnol Prog
; 23(2): 359-63, 2007.
Article
en En
| MEDLINE
| ID: mdl-17253724
ABSTRACT
The influence of Triton X-100 on Beta vulgaris L. permeabilized cell culture viability, regrowth, and ability to produce betacyanines was evaluated in this study. A non-destructive method based on the analysis of images in the RGB (red, green, blue) system was developed to estimate betacyanines content. A treatment for 15 min with 0.7 mM Triton X-100 induced the release of 30% of betacyanines without loss of cell viability (>or=70%). After this permeabilization treatment, B. vulgaris cultures regrew normally, reaching a maximum biomass concentration of 48% higher than non-permeabilized cultures after 14 days of culture. Also, maximum betacyanines concentration was only 25% lower than that of non-permeabilized cultures.
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Banco de datos:
MEDLINE
Asunto principal:
Octoxinol
/
Colorimetría
/
Técnicas de Cultivo de Célula
/
Beta vulgaris
/
Betacianinas
/
Microscopía Fluorescente
Idioma:
En
Año:
2007
Tipo del documento:
Article