Unexpected altered specificity is responsible for St. Louis encephalitis virus recombinant protease autoproteolysis.

Pastorino, Boris A M; Peyrefitte, Christophe N; Grandadam, Marc; Lebrun, Régine; Moinier, Danielle; Rolland, Dominique; Tolou, Hugues J; Bessaud, Maël

Pastorino, Boris A M; Peyrefitte, Christophe N; Grandadam, Marc; Lebrun, Régine; Moinier, Danielle; Rolland, Dominique; Tolou, Hugues J; Bessaud, Maël.

Pastorino BA; Unité de virologie tropicale, Institut de médecine tropicale du service de santé des armées, BP 46, 13 998 Marseille armées, France. publi.viro@laposte.net

Protein Pept Lett ; 14(1): 79-82, 2007.

Article en En | MEDLINE | ID: mdl-17266653

ABSTRACT

ABSTRACT

We report herein the study of the cleavage fragments generated by autoproteolysis of the St. Louis encephalitis virus recombinant protease. The cleavage sites leading to truncated forms were identified by microsequencing, which revealed an unexpected altered specificity of the recombinant proteinase towards unusual sequences.

Asunto(s)

Virus de la Encefalitis de San Luis/enzimología; Endopeptidasas/metabolismo; Proteínas Recombinantes/metabolismo; Secuencia de Aminoácidos; Datos de Secuencia Molecular; Especificidad por Sustrato

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Banco de datos: MEDLINE Asunto principal: Endopeptidasas / Proteínas Recombinantes / Virus de la Encefalitis de San Luis Idioma: En Año: 2007 Tipo del documento: Article

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PubMed Links

Banco de datos: MEDLINE Asunto principal: Endopeptidasas / Proteínas Recombinantes / Virus de la Encefalitis de San Luis Idioma: En Año: 2007 Tipo del documento: Article