The role of estrogen receptor subtypes in ameliorating hepatic injury following trauma-hemorrhage.
J Hepatol
; 46(6): 1047-54, 2007 Jun.
Article
en En
| MEDLINE
| ID: mdl-17336418
ABSTRACT
BACKGROUND/AIMS:
The aim of this study was to determine which of the estrogen receptor (ER) subtypes plays a predominant role in ameliorating hepatic damage following trauma-hemorrhage.METHODS:
Adult male rats were subjected to hemorrhagic shock (40 mmHg for 90 min) and resuscitation. ER-alpha agonist (PPT) or ER-beta agonist (DPN) was administered during resuscitation; rats were sacrificed 24h thereafter.RESULTS:
PPT or DPN decreased elevated plasma alpha-glutathione S-transferase levels; however, PPT was more effective. PPT or DPN increased hepatic heat shock protein 32 (Hsp32) mRNA/protein expressions above levels observed after trauma-hemorrhage. PPT reduced hepatic NF-kappaB and AP-1 activity and iNOS expression. Although DPN reduced hepatic NF-kappaB activity, AP-1 activity remained higher than in shams; hepatic iNOS induction remained elevated. PPT/DPN reduced nitrate/nitrite production and iNOS mRNA in Kupffer cells following trauma-hemorrhage; however, these levels in DPN-treated animals remained higher than sham.CONCLUSIONS:
Although both PPT and DPN decreased hepatic injury following trauma-hemorrhage, ER-alpha agonist PPT appears to be more effective in downregulating NF-kappaB and AP-1 activity, and iNOS induction. Thus, ER-alpha appears to play a predominant role in mediating the salutary effects of E2 in ameliorating hepatic damage following trauma-hemorrhage.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Receptores de Estrógenos
/
Hemorragia
/
Hígado
Límite:
Animals
Idioma:
En
Año:
2007
Tipo del documento:
Article