[Molecular genetic methods of typing of the Bartonellae].
Mol Gen Mikrobiol Virusol
; (1): 8-15, 2007.
Article
en Ru
| MEDLINE
| ID: mdl-17354603
ABSTRACT
The primer systems for the PCR detection of four house-keeping genes of bartonellae in clinical material were developed and tested. The tactics of the species RFLP typing was also developed and tested. The scheme of the species RFLP typing of bartonellae was tested using as an example two strains for the first time isolated in Russia from patients with endocarditis and fever of uncertain origin. The results of the typing were supported by sequencing of the amplicons obtained. According to the sequencing the isolates were attributed to the sub species Bartonella vinsonii, subsp. arupensis. The necessity of molecular epidemiological analysis of bartonelloses in Russia was substantiated.
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Banco de datos:
MEDLINE
Asunto principal:
Bartonella
/
Polimorfismo de Longitud del Fragmento de Restricción
/
Reacción en Cadena de la Polimerasa
/
Técnicas de Tipificación Bacteriana
/
Biología Molecular
Tipo de estudio:
Evaluation_studies
Idioma:
Ru
Año:
2007
Tipo del documento:
Article