[Observation on the ultrastructure of Pneumocystis carinii].
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi
; 24(6): 449-52, 2006 Dec.
Article
en Zh
| MEDLINE
| ID: mdl-17366977
ABSTRACT
OBJECTIVE:
To study the life cycle and morphology of Pneumocystis carinii by ultrastructural observation.METHODS:
Wistar rat model of P. carinii infection was established by subcutaneous injection with dexamethasone. Lung tissue of the infected rats was used for the transmission electron microscopical study.RESULTS:
The organisms were mainly present in the lung alveolar cavity, and also in the alveolar septum, pulmonary macrophages and neutrophils. More trophozoites of P. carinii attached to the type I alveolar epithelial cells, and rarely to the type II alveolar epithelial cells. Most of these trophozoites showed pseudopodial evaginations on their pellicles. The nucleus-associated organelle and spindle microtubules were observed in some trophozoites. The precyst phase was in three forms early, intermediate and late form. Synaptonemal complexes indicating meiotic nuclear divisions and a clump of mitochondria were also observed in the precyst. The pellicle of the cyst has a thickened portion with a pore. There were nucleus with nucleolus, mitochondrion, vesicles, endoplasmic reticulum and numerous ribosomes in the organisms, and tubular expansions on its surface.CONCLUSION:
The life cycle of P. carinii consists of trophozoite, precyst and cyst stages. The presence of a single pore in the cyst wall reveals that pore formation may be a mode of excystation for intracystic bodies of P. carinii.
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Banco de datos:
MEDLINE
Asunto principal:
Neumonía por Pneumocystis
/
Alveolos Pulmonares
/
Pneumocystis carinii
Límite:
Animals
Idioma:
Zh
Año:
2006
Tipo del documento:
Article