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Impact of 3,4-dideoxyglucosone-3-ene (3,4-DGE) on cytotoxicity of acidic heat-sterilized peritoneal dialysis fluid.
Tomo, Tadashi; Okabe, Eiji; Yamamoto, Takashi; Namoto, Shinji; Iwashita, Tomohiko; Matsuyama, Kazuhiro; Kadota, Jun-ichi.
  • Tomo T; Second Department of Internal Medicine, Faculty of Medicine, Oita University, Yufu, Oita, Japan. tomo@med.oita-u.ac.jp
J Artif Organs ; 10(1): 47-51, 2007.
Article en En | MEDLINE | ID: mdl-17380297
ABSTRACT
Of the glucose degradation products (GDPs) in glucose-rich peritoneal dialysate, we investigated the influence of 3,4-dideoxyglucosone-3-ene (3,4-DGE) on the cytotoxicity of acidic heat-sterilized peritoneal dialysis fluid (L-H PDF) using human peritoneal mesothelial cells (HPMC). We prepared acidified filtration-sterilized PDF (glucose concentration 3.86%) containing eight types of added GDP [3,4-DGE, glyoxal (GO), methylglyoxal (MGO), 3-deoxyglucosone (3-DG), formaldehyde (FA), acetaldehyde (AA), 5-hydroxymethyl-2-furaldehyde (5-HMF), and furfural (FF)] or seven types of GDP (GO, MGO, 3-DG, FA, AA, 5-HMF, and FF). HPMC were exposed to these two types of solution and acidic heat-sterilized PDF (glucose concentration 3.86%, L-H 3.86) for 4 h. Cell viability was determined by 3,(4,5-dimethythiazol-2-yl)2,5-diphenyl-terazolium bromide (MTT) assay. MTT viability was decreased significantly compared with the control when treated with L-H 3.86 or acidified neutral filtration-sterilized PDF (glucose concentration 3.86%) containing eight GDPs. However, no significant decrease in MTT viability was observed when HPMC were treated with acidified neutral filtration-sterilized PDF (glucose concentration 3.86%) containing seven GDPs. Thus, 3,4-DGE strongly affects the cytotoxicity of L-H PDF. It is suggested that the cytotoxicity of L-H PDF is based on the presence of 3,4-DGE.
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Banco de datos: MEDLINE Asunto principal: Pironas / Soluciones para Diálisis / Soluciones para Hemodiálisis Límite: Humans Idioma: En Año: 2007 Tipo del documento: Article
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Banco de datos: MEDLINE Asunto principal: Pironas / Soluciones para Diálisis / Soluciones para Hemodiálisis Límite: Humans Idioma: En Año: 2007 Tipo del documento: Article