Assessment of fetomaternal hemorrhage by flow cytometry and Kleihauer-Betke test in Rh-negative pregnancies.
Gynecol Obstet Invest
; 65(2): 84-8, 2008.
Article
en En
| MEDLINE
| ID: mdl-17878734
ABSTRACT
OBJECTIVES:
To assess the efficacy of flow cytometry (FC) in the detection and quantification of fetomaternal hemorrhage (FMH) in comparison to the Kleihauer-Betke test (KBT).METHODS:
25 unsensitized Rh-negative mothers who had delivered Rh-positive infants were included. Presence of FMH was determined by KBT and FC using FITC-labeled BRAD-3 antibodies.RESULTS:
FMH was detected in 19 (76%) patients by FC and 23 (92%) patients by KBT prior to delivery, and in 21 (84%) patients by FC and 23 (92%) patients by KBT after delivery. The mean volume of FMH in the post-delivery samples by KBT and FC were 0.34 +/- 0.26 ml (range 0.05-1.2 ml) and 0.37 +/- 0.57 ml (range 0.02-2.6 ml) respectively. The volume of post-delivery FMH estimated by KBT and FC correlated well (r = 0.75; ICC alpha = 0.73). A higher agreement between KBT and FC was seen in the 0.1-0.5 ml range (kappa = 0.65; p < 0.01).CONCLUSIONS:
Both manual KBT and FC using FITC-BRAD-3 antibodies show good sensitivity in detecting and quantifying fetal red cells. There is a good correlation between the methods in the 0.1- to 0.5-ml range of FMH.
Search on Google
Banco de datos:
MEDLINE
Asunto principal:
Sistema del Grupo Sanguíneo Rh-Hr
/
Transfusión Fetomaterna
/
Citometría de Flujo
/
Anticuerpos Monoclonales
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Adult
/
Female
/
Humans
/
Newborn
/
Pregnancy
Idioma:
En
Año:
2008
Tipo del documento:
Article