Maintenance of surrogate light chain expression induces developmental delay in early B cell compartment.
J Immunol
; 179(8): 4996-5005, 2007 Oct 15.
Article
en En
| MEDLINE
| ID: mdl-17911584
ABSTRACT
The production of a mature B cell requires passage through a number of developmental checkpoints. The pre-BCR plays a critical role in passage through the pro-B cell/pre-B cell checkpoint, and thus plays a central role in regulating the differentiation of a B cell. Due to the significance of this receptor, it is imperative that pre-BCR expression and function are precisely regulated. In this study, we have investigated a system in which the regulation of the pre-BCR is altered. We have found that continued expression of components of the pre-BCR (lambda5) resulted in a delay in the kinetics of B cell maturation. Pro-B cells from normal mouse bone marrow retrovirally infected with lambda5 exhibited a delay in differentiation. As compared with wild-type cells at the same time point, there is a reduction in the presence of cell surface markers that indicate developmental progression, and there is a 6- to 16-fold decrease in the production of Ig-positive cells in B cell maturation assays. The capacity to alter B cell progression by modifying and extending pre-BCR expression argues that the receptor and its associated signals play a unique role in directing developmental outcomes.
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Banco de datos:
MEDLINE
Asunto principal:
Diferenciación Celular
/
Subgrupos de Linfocitos B
/
Inmunoglobulina de Cadenas Ligeras Subrogadas
/
Inhibidores de Crecimiento
Límite:
Animals
Idioma:
En
Año:
2007
Tipo del documento:
Article