[Experimental gene therapy of keloid in vivo using recombinant adenovirus carrying Fas gene].

ABSTRACT

OBJECTIVE: To observe the gene therapeutic effects of two kinds of prepared recombinant adenovirus coding for Fas gene on keloid in nude mouse model. METHODS: Keloid tissue was transplanted into athymic nude mice subcutaneously to establish the animal model of keloid. Forty-five days after the model had been established, the recombinant adenovirus vectors carrying Fas gene [Ad-Fas (B) or Ad-Fas (T)] or empty vector were injected into the keloid tissues. One day after that, anti-Fas monoclonal antibody (FasMcab) was injected in the keloid tissues. And 7 days after, the animals were sacrificed and changes of the keloid tissues were observed generally and by pathological and electronic microscopic examinations. RESULTS: The keloid tissues shrunk slightly in the animals injected adenovirus only. The keloid tissues diminished significantly in the animals after injection of Ad-Fas (T) or Ad-Fas (B) followed by FasMcab. Destroyed structure of keloid tissue was found with HE stain. Obvious apoptosis was testified with the electronic microscope. CONCLUSIONS: Gene therapy of keloid in vivo with recombinant adenovirus carrying Fas gene is effective, and it maybe a potential therapy for keloid.