Genome organization and reverse genetic analysis of a type I feline coronavirus.
J Virol
; 82(4): 1851-9, 2008 Feb.
Article
en En
| MEDLINE
| ID: mdl-18077720
ABSTRACT
In this study we report the complete sequence and genome organization of the serotype I feline coronavirus (FCoV) strain Black. Furthermore, a reverse genetic system was established for this FCoV strain by cloning a full-length cDNA copy into vaccinia virus. This clone served as basis for the generation of recombinant FCoV (recFCoV) that was shown to bear the same features in vitro as the parental FCoV. Using this system, accessory 3abc genes in the FCoV genome were replaced by green fluorescent protein (recFCoV-GFP) and Renilla luciferase genes (recFCoV-RL). In addition, we showed that feline CD14(+) blood monocytes and dendritic cells can be easily detected after infection with recFCoV-GFP. Thus, our established reverse genetic system provides a suitable tool to study the molecular biology of serotype I FCoV.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Genoma Viral
/
Análisis de Secuencia de ADN
/
Coronavirus Felino
Límite:
Animals
Idioma:
En
Año:
2008
Tipo del documento:
Article