A microwave technique for double indirect immunostaining of human brain tissue cultures with mouse monoclonal antibodies.
Appl Immunohistochem Mol Morphol
; 16(1): 83-6, 2008 Jan.
Article
en En
| MEDLINE
| ID: mdl-18091314
ABSTRACT
The use of 2 monoclonal antibodies during double immunohistochemistry would enable the use of a greater variety of antibody combinations. Here, we demonstrate a simple, cost effective method of double indirect immunostaining of cultured cells using primary antibodies from the same species. This method uses microwaving of cell samples immediately after the application of the first secondary antibody, and significantly reduces the level of nonspecific staining. This technique does not elute the antibodies, nor raise the sample temperature above 37 degrees C.
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Banco de datos:
MEDLINE
Asunto principal:
Tubulina (Proteína)
/
Química Encefálica
/
Inmunohistoquímica
/
Técnicas para Inmunoenzimas
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Proteína Ácida Fibrilar de la Glía
/
Microondas
/
Anticuerpos Monoclonales
Límite:
Animals
/
Humans
Idioma:
En
Año:
2008
Tipo del documento:
Article