[Response of glycine and glycyl-L-valine uptake parametrs across in vitro everted sacs of chicken gut to variations in oxygenation rate and fasting].
Ross Fiziol Zh Im I M Sechenova
; 93(12): 1423-34, 2007 Dec.
Article
en Ru
| MEDLINE
| ID: mdl-18318182
ABSTRACT
Energy-dependent accumulation of glycine and glycyl-L-valine within the small intestinal mucosa in a chicken model of in vitro local oxygenation of the small intestinal preparation was studied. It has been shown that the most effective bilateral oxygenation significantly increase accumulation of glycyl-L-valine in the proximal segment as compared to that under oxygenation only from serosal surface both in the fed and 24-hour fasted chickens, whereas in other segments these differences was less apparent. This may be due to increased H+/ peptide cotransporter expression in the proximal segment. Thus the bilateral oxygenation probably may turn on an additional amount of already existing (but non-functional during serosal oxygenation) H+/ peptide co-transporters. Moreover, low glycine transporter expression may be the reason why supplemental oxygen (bilateral oxygenation) has no effect on glycine accumulation in the distal segment of fed chickens. A 48-hour fasting decreases glycyl-L-valine accumulation in the proximal (and medial) segments, possibly due to progressive decrease in villus height. It is concluded that a) the accumulation rate of glycine was greater when presented as the glycyl-L-valine than when presented as the equivalent amount of free amino acid; b) the rates of accumulation of glycyl-L-valine are highest in the proximal segment, decrease in the medial segment and are the lowest in the distal segment; c) the serosal oxygenation is less effective than the mucosal and bilateral oxygenation, which markedly stimulates accumulation of nutrients in the intestinal mucosa; d) a 24-hour fasting increases glycyl-L-valine accumulation in the proximal segment only, while glycine uptake was increased in the distal segment.
Search on Google
Banco de datos:
MEDLINE
Asunto principal:
Ayuno
/
Dipéptidos
/
Glicina
/
Absorción Intestinal
/
Intestino Delgado
Límite:
Animals
Idioma:
Ru
Año:
2007
Tipo del documento:
Article