AFM imaging of RGD presenting synthetic extracellular matrix using gold nanoparticles.
Macromol Biosci
; 8(6): 469-77, 2008 Jun 11.
Article
en En
| MEDLINE
| ID: mdl-18383570
ABSTRACT
Several high-resolution imaging techniques such as FESEM, TEM and AFM are compared with respect to their application on alginate hydrogels, a widely used polysaccharide biomaterial. A new AFM method applicable to RGD peptides covalently conjugated to alginate hydrogels is described. High-resolution images of RGD adhesion ligand distribution were obtained by labeling biotinylated RGD peptides with streptavidin-labeled gold nanoparticles. This method may broadly provide a useful tool for sECM characterization and design for tissue regeneration strategies.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Oligopéptidos
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Microscopía de Fuerza Atómica
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Matriz Extracelular
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Nanopartículas del Metal
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Oro
Idioma:
En
Año:
2008
Tipo del documento:
Article