Screening and identification of differentially expressed genes in myocardial ischaemia/reperfusion injury using suppression subtractive hybridization.
Acta Cardiol
; 63(2): 213-9, 2008 Apr.
Article
en En
| MEDLINE
| ID: mdl-18468203
ABSTRACT
OBJECTIVE:
Myocardial ischaemia/reperfusion (MI/R) injury is characterized by metabolic and ultrastructural changes, which lead to irreversible injury. Several mechanisms have been postulated for the pathogenesis of MI/R injury although little is known regarding the role of myocardial gene expression. METHODS ANDRESULTS:
In this study, suppression subtractive hybridization (SSH) was employed to systematically isolate and screen the differentially expressed genes in the MI/R injury rat model. The characteristics of these specific genes were analysed using bioinformatics. Our results showed that among the 119 identified genes, 54 genes were expressed at higher levels and 65 genes were at lower levels compared with the control group.CONCLUSIONS:
These genes are closely associated with energy metabolism, iron transport, signalling transduction and may provide important clues for the elucidation of the mechanisms of MI/R injury. Our results further indicated that myocardial injury is likely the result of summation of functional impairment of multiple genes rather than the result of damage to a single critical gene.
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Banco de datos:
MEDLINE
Asunto principal:
ARN
/
Daño por Reperfusión Miocárdica
/
Expresión Génica
/
Proteínas de Ciclo Celular
/
Perfilación de la Expresión Génica
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Límite:
Animals
Idioma:
En
Año:
2008
Tipo del documento:
Article