The association of thymidine kinase activity and thymidine transport in Escherichia coli.
Gene
; 99(1): 25-9, 1991 Mar 01.
Article
en En
| MEDLINE
| ID: mdl-1850708
ABSTRACT
We have constructed a series of mutants within the putative nucleoside-binding site of the herpes simplex type-1 virus (HSV-1) thymidine kinase (TK)-encoding gene (tk), contained within an expression vector. While most mutations within this sequence produce an inactive protein, we find no absolute requirement for the wild-type Ile166 and Ala167. The uptake of thymidine (dT) into Escherichia coli tdk-, lacking functional endogenous TK activity, is proportional to the amount of TK activity expressed from the heterologous HSV-1 tk gene. In contrast, there is no enhancement in deoxycytidine uptake into E. coli producing (HSV-1) TK. These results imply a specific role for TK in the active transport of dT into E. coli.
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Banco de datos:
MEDLINE
Asunto principal:
Timidina
/
Timidina Quinasa
/
Simplexvirus
/
Escherichia coli
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Año:
1991
Tipo del documento:
Article