Retroviral vectors related to the myeloproliferative sarcoma virus allow efficient expression in hematopoietic stem and precursor cell lines, but retroviral infection is reduced in more primitive cells.
Hum Gene Ther
; 2(1): 61-70, 1991.
Article
en En
| MEDLINE
| ID: mdl-1863641
ABSTRACT
Retroviral vectors are considered to be the most suited vehicles for somatic gene therapy with hematopoietic stem cells as targets. Retrovirus-mediated gene transfer into differentiation-restricted hematopoietic precursor (FDC-P1, FDC-P2) and multipotent progenitor (stem) cell lines (FDC-Pmix) is inefficient. Two cellular restrictions are involved. One is specific for stem but not precursor cells and is at the level of transcription. Due to a unique property of the transcriptional control region of the myeloproliferative sarcoma virus (MPSV), vectors derived from MPSV are not affected by this block. The second restriction occurs before proviral DNA synthesis and integration. This inhibition of effective viral infection depends on the state of differentiation, being more pronounced in multipotent clonogenic blast cells. This block to retroviral infection affects all retroviral vectors tested.
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Banco de datos:
MEDLINE
Asunto principal:
Células Madre Hematopoyéticas
/
Transfección
/
Regulación de la Expresión Génica
/
Virus Defectuosos
/
Vectores Genéticos
/
Virus del Sarcoma Murino de Moloney
Límite:
Animals
Idioma:
En
Año:
1991
Tipo del documento:
Article