Nuclear localization of SNARK; its impact on gene expression.
Biochem Biophys Res Commun
; 377(4): 1062-6, 2008 Dec 26.
Article
en En
| MEDLINE
| ID: mdl-18992219
ABSTRACT
SNARK, a member of the AMPK-related kinases, has been involved in the cellular stress responses but its precise mechanisms remain unclear. Subcellular localization of SNARK protein was identified. Unlike cytoplasmic localizing AMPKalpha, SNARK was predominantly localized in the nucleus. SNARK was constitutively distributed in the nucleus even when SNARK was activated by metabolic stimuli such as AICAR and glucose-deprivation. Conserved nuclear localization signal (NLS) was identified at the N-terminal portion ((68)KKAR(71)). Deletion and point mutation of this part resulted in the cytoplasmic translocation of mutant proteins. Furthermore, GFP fused with the SNARK fragment containing (68)KKAR(71) translocated to the nucleus. A microarray analysis revealed that the nuclear localizing SNARK altered transcriptome profiles and a considerable part of these alterations were canceled by the mutation of NLS, suggesting the ability of SNARK to modulate gene expression dependent on its nuclear localization.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Núcleo Celular
/
Regulación de la Expresión Génica
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Proteínas Serina-Treonina Quinasas
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Señales de Localización Nuclear
Límite:
Humans
Idioma:
En
Año:
2008
Tipo del documento:
Article