The cell adhesion molecule Tag1, transmembrane protein Stbm/Vangl2, and Lamininalpha1 exhibit genetic interactions during migration of facial branchiomotor neurons in zebrafish.
Dev Biol
; 325(2): 363-73, 2009 Jan 15.
Article
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| MEDLINE
| ID: mdl-19013446
ABSTRACT
Interactions between a neuron and its environment play a major role in neuronal migration. We show here that the cell adhesion molecule Transient Axonal Glycoprotein (Tag1) is necessary for the migration of the facial branchiomotor neurons (FBMNs) in the zebrafish hindbrain. In tag1 morphant embryos, FBMN migration is specifically blocked, with no effect on organization or patterning of other hindbrain neurons. Furthermore, using suboptimal morpholino doses and genetic mutants, we found that tag1, lamininalpha1 (lama1) and stbm, which encodes a transmembrane protein Vangl2, exhibit pairwise genetic interactions for FBMN migration. Using time-lapse analyses, we found that FBMNs are affected similarly in all three single morphant embryos, with an inability to extend protrusions in a specific direction, and resulting in the failure of caudal migration. These data suggest that tag1, lama1 and vangl2 participate in a common mechanism that integrates signaling between the FBMN and its environment to regulate migration.
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1
Banco de datos:
MEDLINE
Asunto principal:
Rombencéfalo
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Pez Cebra
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Moléculas de Adhesión Celular Neuronal
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Laminina
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Proteínas de Pez Cebra
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Proteínas de la Membrana
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Neuronas Motoras
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Animals
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En
Año:
2009
Tipo del documento:
Article