Relationship of the uptake and beta-oxidation of 18-carbon fatty acids with stimulation of murine mammary tumor cell growth.

ABSTRACT

The effects of stearic (180), oleic (181) and linoleic (182) acid on the in vitro growth of murine mammary tumor cell line 4526 were compared to 4526 uptake and beta-oxidation of those fatty acids. Both 181 and 182 stimulated, while 180 inhibited growth. Likewise, uptake and oxidation rates were much lower for 180 than for 181 and 182. The influence of glucose, insulin and fatty acid-depletion on 4526 fatty acid incorporation and oxidation was also determined. The correlation between effects on in vitro growth and ability to assimilate 180, 181 and 182 may partially explain the stimulation by unsaturated and inhibition by saturated fatty acids of in vivo 4526 tumorigenesis.