Active mask segmentation of fluorescence microscope images.
IEEE Trans Image Process
; 18(8): 1817-29, 2009 Aug.
Article
en En
| MEDLINE
| ID: mdl-19380268
ABSTRACT
We propose a new active mask algorithm for the segmentation of fluorescence microscope images of punctate patterns. It combines the (a) flexibility offered by active-contour methods, (b) speed offered by multiresolution methods, (c) smoothing offered by multiscale methods, and (d) statistical modeling offered by region-growing methods into a fast and accurate segmentation tool. The framework moves from the idea of the "contour" to that of "inside and outside," or masks, allowing for easy multidimensional segmentation. It adapts to the topology of the image through the use of multiple masks. The algorithm is almost invariant under initialization, allowing for random initialization, and uses a few easily tunable parameters. Experiments show that the active mask algorithm matches the ground truth well and outperforms the algorithm widely used in fluorescence microscopy, seeded watershed, both qualitatively, as well as quantitatively.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Algoritmos
/
Procesamiento de Imagen Asistido por Computador
/
Reconocimiento de Normas Patrones Automatizadas
/
Microscopía Fluorescente
Límite:
Humans
Idioma:
En
Año:
2009
Tipo del documento:
Article