A physical and functional link between splicing factors promotes pre-mRNA 3' end processing.
Nucleic Acids Res
; 37(14): 4672-83, 2009 Aug.
Article
en En
| MEDLINE
| ID: mdl-19506027
ABSTRACT
Polypyrimidine tract-binding protein (PTB) is a splicing regulator that also plays a positive role in pre-mRNA 3' end processing when bound upstream of the polyadenylation signal (pA signal). Here, we address the mechanism of PTB stimulatory function in mRNA 3' end formation. We identify PTB as the protein factor whose binding to the human beta-globin (HBB) 3' UTR is abrogated by a 3' end processing-inactivating mutation. We show that PTB promotes both in vitro 3' end cleavage and polyadenylation and recruits directly the splicing factor hnRNP H to G-rich sequences associated with several pA signals. Increased binding of hnRNP H results in stimulation of polyadenylation through a direct interaction with poly(A) polymerase. Therefore, our results provide evidence of a concerted regulation of pA signal recognition by splicing factors bound to auxiliary polyadenylation sequence elements.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
ARN Mensajero
/
Precursores del ARN
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Procesamiento de Término de ARN 3'
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Proteína de Unión al Tracto de Polipirimidina
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Globinas beta
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Año:
2009
Tipo del documento:
Article