Production of an egg yolk antibody against Parietaria judaica 2 allergen.
Poult Sci
; 88(8): 1773-8, 2009 Aug.
Article
en En
| MEDLINE
| ID: mdl-19590094
ABSTRACT
Specific antibodies are essential tools for studying proteins as well as for diagnostic research in biomedicine. The egg yolk of immunized chicken is an inexpensive source of high-quality polyclonal antibodies. The 12-kDa Parietaria judaica 2 allergen was expressed as a fusion protein and was used to immunize Leghorn chickens. In this paper, we show, using 2-dimensional gel electrophoresis and immunoblotting, that chicken antibodies raised against a recombinant allergen can be used to recognize similar proteins from a pollen raw extract. Allergen identity was confirmed by nanoLC-nanospray-tandem mass spectrometry analysis. Our data demonstrate for the first time that a synergistic combination of molecular biology, 2-dimensional PAGE, and use of nonmammalian antibodies represents a powerful tool for reliable identification of allergens.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Inmunoglobulinas
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Pollos
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Parietaria
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Yema de Huevo
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Antígenos de Plantas
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Anticuerpos
Límite:
Animals
Idioma:
En
Año:
2009
Tipo del documento:
Article