Subcellular localization of Trypanosoma cruzi arginine kinase.
Parasitology
; 136(10): 1201-7, 2009 Sep.
Article
en En
| MEDLINE
| ID: mdl-19631011
ABSTRACT
Phosphoarginine is a cell energy buffer molecule synthesized by the enzyme arginine kinase. In Trypanosoma cruzi, the aetiological agent of Chagas' disease, 2 different isoforms were identified by data mining, but only 1 was expressed during the parasite life cycle. The digitonin extraction pattern of arginine kinase differed from those obtained for reservosomes, glycosomes and mitochondrial markers, and similar to the cytosolic marker. Immunofluorescence analysis revealed that although arginine kinase is localized mainly in unknown punctuated structures and also in the cytosol, it did not co-localize with any of the subcelular markers. This punctuated pattern has previously been observed in many cytosolic proteins of trypanosomatids. The knowledge of the subcellular localization of phosphagen kinases is a crucial issue to understand their physiological role in protozoan parasites.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Arginina Quinasa
/
Fracciones Subcelulares
/
Trypanosoma cruzi
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Año:
2009
Tipo del documento:
Article